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Omega Bio Tek ezna hp plant mini kit
Ezna Hp Plant Mini Kit, supplied by Omega Bio Tek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ezna+hp+plant+mini+kit/ezna+hp+plant+mini+kit/pm40647985-164-17-22
Average 90 stars, based on 1 article reviews
ezna hp plant mini kit - by Bioz Stars, 2026-09
90/100 stars

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DNA Extraction:

Article Title: Phylogenetic inference enables reconstruction of a long-overlooked outbreak of almond leaf scorch disease ( Xylella fastidiosa ) in Europe
Article Snippet: .. DNA extraction from plant extracts was performed using an EZNA HP Plant Mini kit (Omega-Biotek, Norcross, Georgia, USA) based on CTAB, as described in the EPPO protocol , whereas Xf genomic DNA was extracted using the Wizard DNA Promega kit. .. DNA extracts were tested for the presence of Xf by qPCR using two species-specific protocols with primers XF-F/XF-R and the dual-labelled probe XF-P (Harper et al. ) and primers HL5/HL6 and a TaqMan probe (Francis et al. ) using an ECO thermal cycler (PCRmax, Staffordshire, UK).

Article Title: Xylem Sap Mycobiota in Grapevine Naturally Infected with Xylella fastidiosa : A Case Study: Interaction of Xylella fastidiosa with Sclerotinia sclerotiorum .
Article Snippet: .. DNA extraction from plant extracts was performed from leaf veins and petioles, and xylem sap using an EZNA HP Plant Mini kit (Omega-Biotek, Norcross, GA, USA) following the manufacturer’s instructions, as described in the EPPO protocol (EPPO, 2016) [45]. .. The presence of Xff was assessed by real-time PCR using two specific proto- cols with primers XF-F/XF-R and the TaqMan probe XF-P (Harper et al., 2010) [46] and primers HL5/HL6 and the TaqMan probe HL-P (Francis et al., 2008) [21] using an Applied Biosystems QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) [21,46].

Article Title: Insights into the epidemiology of Pierce's disease in vineyards of Mallorca, Spain
Article Snippet: .. DNA extraction from plant extracts was performed using an EZNA HP Plant Mini kit (Omega-Biotek, Norcross, Georgia, USA), based on CTAB, as described in the EPPO protocol (EPPO, 2018), whereas DNA extractions from bacteria and vectors were based on CTAB protocol. .. DNA plant extracts were tested for the presence of Xf by Taqman probe real-time PCR using an ECO thermal cycler (PCRmax, Staffordshire, UK) and two species-specific protocols (EPPO, 2018) were used for the detection and identification of Xf (Harper et al., 2010, erratum 2013 and Francis et al., 2006).

Extraction:

Article Title: Use of traC Gene to Type the Incidence and Distribution of pXFAS_5235 Plasmid-Bearing Strains of Xylella fastidiosa subsp. fastidiosa ST1 in Spain
Article Snippet: .. Total plant DNA was extracted as described in the EPPO protocol [ ] from leaf petioles or wood chip samples using the cetyltrimethylammonium bromide (CTAB)-based extraction or the EZNA HP Plant Mini kit (Omega-Biotek, Norcross, Georgia, USA), depending on the official laboratory providing the samples. ..

Isolation:

Article Title: Comparative Chloroplast Genome Analyses of Streptophyte Green Algae Uncover Major Structural Alterations in the Klebsormidiophyceae, Coleochaetophyceae and Zygnematophyceae
Article Snippet: .. Total cellular DNA from Cosmarium and Cylindrocystis was isolated using the EZNA HP Plant Mini Kit of Omega Bio-Tek (Norcross, GA, USA). .. For Illumina sequencing of the Closterium, Cosmarium , and Cylindrocystis chloroplast genomes, libraries of 700-bp fragments were constructed using the TrueSeq DNA Sample Prep Kit (Illumina, San Diego, CA, USA) and paired-end reads were generated on the Illumina HiSeq 2000 (100-bp reads) or the MiSeq (300-bp reads) sequencing platforms by the Innovation Centre of McGill University and Génome Québec and the “Plateforme d’Analyses Génomiques de l’Université Laval ,” respectively.

Article Title: Divergent copies of the large inverted repeat in the chloroplast genomes of ulvophycean green algae
Article Snippet: Purified PCR products were sequenced using Sanger chemistry with the PRISM BigDye Terminator Ready Reaction Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) on ABI model 373 or 377 DNA sequencers (Applied Biosystems). .. For Illumina sequencing, total cellular DNA was isolated using the EZNA HP Plant Mini Kit of Omega Bio-Tek (Norcross, GA, USA). .. Libraries of 500-bp fragments were constructed using the TrueSeq DNA Sample Prep Kit (Illumina, San Diego, CA, USA) and paired-end reads were generated on the Illumina HiSeq 2000 (100-bp reads) or the MiSeq (300-bp reads) sequencing platforms by the Innovation Centre of McGill University and Génome Québec ( http://gqinnovationcenter.com/index.aspx ) and the “Plateforme d’Analyses Génomiques de l’Université Laval”, respectively.

Article Title: Mitochondrion-to-Chloroplast DNA Transfers and Intragenomic Proliferation of Chloroplast Group II Introns in Gloeotilopsis Green Algae (Ulotrichales, Ulvophyceae)
Article Snippet: The G. sarcinoidea organelle genomes were sequenced using the Illumina method. .. Total cellular DNA was isolated using the EZNA HP Plant Mini Kit of Omega Bio-Tek (Norcross, GA, USA). .. A library of 500-bp fragments was constructed using the TrueSeq DNA Sample Prep Kit (Illumina, San Diego, CA, USA) and paired-end reads (300-bp) were generated on the MiSeq sequencer by the “Plateforme d’Analyses Génomiques de l’Université Laval” ( http://pag.ibis.ulaval.ca/seq/en/index.php ; last accessed 8 August 2016).

Sterility:

Article Title: Studies on the competence of potential Xylella fastidiosa vectors in the Balearic Islands (Spain)
Article Snippet: .. 0,5g of petioles and midribs in 5ml of sterile PBS homogenized using a Homex - were performed using EZNA HP Plant Mini Kit (Omega - Biotek) meanwhile CTAB protocol (EPPO, 2018) was followed for insect vectors, prior homogenization of single heads using the Tissue Lyser II (QIA-GEN). ..

Homogenization:

Article Title: Studies on the competence of potential Xylella fastidiosa vectors in the Balearic Islands (Spain)
Article Snippet: .. 0,5g of petioles and midribs in 5ml of sterile PBS homogenized using a Homex - were performed using EZNA HP Plant Mini Kit (Omega - Biotek) meanwhile CTAB protocol (EPPO, 2018) was followed for insect vectors, prior homogenization of single heads using the Tissue Lyser II (QIA-GEN). ..

Bacteria:

Article Title: Insights into the epidemiology of Pierce's disease in vineyards of Mallorca, Spain
Article Snippet: .. DNA extraction from plant extracts was performed using an EZNA HP Plant Mini kit (Omega-Biotek, Norcross, Georgia, USA), based on CTAB, as described in the EPPO protocol (EPPO, 2018), whereas DNA extractions from bacteria and vectors were based on CTAB protocol. .. DNA plant extracts were tested for the presence of Xf by Taqman probe real-time PCR using an ECO thermal cycler (PCRmax, Staffordshire, UK) and two species-specific protocols (EPPO, 2018) were used for the detection and identification of Xf (Harper et al., 2010, erratum 2013 and Francis et al., 2006).



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